Coding
LanM

Part:BBa_K4744000:Design

Designed by: Paul Cordero, Christian Kuehne   Group: iGEM23_Aachen   (2023-10-09)


coding region for Lanmodulin without stop codon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 85
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The stop codon was deleted to allow C-terminal fusion including a his6-tag. The LanM gene was amplified with designed primers to eliminate the stop codon as well as the N-terminal his-tag and indroduce restriction sites to be able to construct it into a vector.



Source

Lanmodulin is abundant in Methylorubrum extorquens. The gene was obtained from a modified pet19b-plasmid containing LanM created by iGEM Bonn 2021.

References

Erik C. Cook, Emily R. Featherston, Scott A. Showalter, and Joseph A. Cotruvo Jr., Structural Basis for Rare Earth Element Recognition by Methylobacterium extorquens Lanmodulin Biochemistry 2019 58 (2), 120-125 DOI: 10.1021/acs.biochem.8b01019