Coding
LanM
Part:BBa_K4744000:Design
Designed by: Paul Cordero, Christian Kuehne Group: iGEM23_Aachen (2023-10-09)
coding region for Lanmodulin without stop codon
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 85
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The stop codon was deleted to allow C-terminal fusion including a his6-tag. The LanM gene was amplified with designed primers to eliminate the stop codon as well as the N-terminal his-tag and indroduce restriction sites to be able to construct it into a vector.
Source
Lanmodulin is abundant in Methylorubrum extorquens. The gene was obtained from a modified pet19b-plasmid containing LanM created by iGEM Bonn 2021.
References
Erik C. Cook, Emily R. Featherston, Scott A. Showalter, and Joseph A. Cotruvo Jr., Structural Basis for Rare Earth Element Recognition by Methylobacterium extorquens Lanmodulin Biochemistry 2019 58 (2), 120-125 DOI: 10.1021/acs.biochem.8b01019